Low intensity near-infrared light promotes bone regeneration via circadian clock protein cryptochrome 1

Low intensity near-infrared light promotes bone regeneration via circadian clock protein cryptochrome 1

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Abstract Bone regeneration remains a great clinical challenge.Low intensity near-infrared (NIR) light showed strong potential to promote tissue regeneration, offering a promising strategy for bone defect regeneration.However, the effect and underlying mechanism Redesign-Transfer of NIR on bone regeneration remain unclear.We demonstrated that bone regeneration in the rat skull defect model was significantly accelerated with low-intensity NIR stimulation.In vitro studies showed that NIR stimulation could promote the osteoblast differentiation in bone mesenchymal stem cells (BMSCs) and MC3T3-E1 cells, which was associated with increased ubiquitination of the core circadian clock protein Cryptochrome 1 (CRY1) in the nucleus.

We found that the reduction of CRY1 induced by NIR light activated the bone morphogenetic protein (BMP) signaling Accessories pathways, promoting SMAD1/5/9 phosphorylation and increasing the expression levels of Runx2 and Osterix.NIR light treatment may act through sodium voltage-gated channel Scn4a, which may be a potential responder of NIR light to accelerate bone regeneration.Together, these findings suggest that low-intensity NIR light may promote in situ bone regeneration in a CRY1-dependent manner, providing a novel, efficient and non-invasive strategy to promote bone regeneration for clinical bone defects.